Answer: Without knowing whether the plants you removed are reputed to be invasive, I can't say with certainty - but if you removed the main root masses of the plants you dug from the bed, the remaining roots will probably die off. Exceptions to this rule are plants such as horseradish and mint. Their roots will sprout if left behind in a bed. If you're unsure about the original plants it will probably be worth your effort to dig down and remove any remaining roots before planting your new bed.
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