Daylilies forum: Converting Diploids to Tetraploids - The Process

Page 1 of 3 • 1 2 3
Views: 3121, Replies: 55 » Jump to the end
Name: Betty
Bakersfield, CA
Charter ATP Member I was one of the first 300 contributors to the plant database! Birds The WITWIT Badge Region: United States of America Roses
Irises Cat Lover Daylilies Region: California Garden Ideas: Level 1
Image
Betja
Nov 26, 2013 12:11 PM CST
Bill Waldrop currently has a wonderful description, complete with pictures, for the actual initial process used in converting diploids to tetraploids.

http://billsdaylilycorner.blogspot.com

After the initial conversion process it is necessary to wait until the plant blooms again so that he can check the pollen with a microscope to see if it has doubled in size, and that is how he confirms that the plant has been converted.

Betty
Name: Theresa Maris
Bowling Green,KY (Zone 6b)
Daylilies Greenhouse Hybridizer Plant and/or Seed Trader Keeper of Poultry Lover of wildlife (Raccoon badge)
Birds Dog Lover Region: Kentucky Hummingbirder Herbs
Image
tgarden711
Nov 26, 2013 5:25 PM CST
Excellent instructions and I understand what he is doing but what is DMSO?
Name: Betty
Bakersfield, CA
Charter ATP Member I was one of the first 300 contributors to the plant database! Birds The WITWIT Badge Region: United States of America Roses
Irises Cat Lover Daylilies Region: California Garden Ideas: Level 1
Image
Betja
Nov 26, 2013 5:26 PM CST
Don't know -- Michele?

Betty
Name: Becky
Sebastian, Florida (Zone 10a)
Celebrating Gardening: 2015 Daylilies Hummingbirder Butterflies Seed Starter Container Gardener
Charter ATP Member I was one of the first 300 contributors to the plant database! Garden Ideas: Master Level Lover of wildlife (Black bear badge) Birds Ponds
Image
beckygardener
Nov 26, 2013 5:30 PM CST
Could this be what DMSO is?
http://en.wikipedia.org/wiki/Dimethyl_sulfoxide

If so ... how does one obtain it?
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
Garden Rooms and Becky's Budget Garden
(Zone 7a)
Garden Ideas: Level 1 Avid Green Pages Reviewer
Image
dormantsrule
Nov 26, 2013 5:34 PM CST
Dimethylsulfoxide. I don't know what grade is used for conversion, but Bill Waldrop would be able to answer.

https://en.wikipedia.org/wiki/Dimethyl_sulfoxide

http://www.ebay.com/bhp/dimethyl-sulfoxide
Photo used in avatar purchased on istockphoto.com
[Last edited by dormantsrule - Nov 26, 2013 5:39 PM (+)]
Give a thumbs up | Quote | Post #518112 (5)
Name: Becky
Sebastian, Florida (Zone 10a)
Celebrating Gardening: 2015 Daylilies Hummingbirder Butterflies Seed Starter Container Gardener
Charter ATP Member I was one of the first 300 contributors to the plant database! Garden Ideas: Master Level Lover of wildlife (Black bear badge) Birds Ponds
Image
beckygardener
Nov 26, 2013 5:38 PM CST
Someone needs to email Bill Waldrop for more indepth details about this!
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
Garden Rooms and Becky's Budget Garden
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
Image
JWWC
Nov 26, 2013 5:47 PM CST
DMSO is typically used for a couple of purposes. It is a pretty powerful solvent used in biology and chemistry. Additionally, for biological purposes it is used as a cell penetrant. It can readily pass through cell membranes and help convey chemicals into cells.
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 26, 2013 6:35 PM CST
We buy it at the feed store; it is used for joint pain in animals and people use it for a wide range of things.

DMSO 99.9% Liquid can be helpful for relieving pain, inflammation, scleroderma, and interstitial cystitis however, intended use is as a solvent only and the choice of using it in other applications is the sole responsibility of the user. DMSO has been used for over 100 years in the United States. It is a natural substance derived from wood pulp and is harmless when used with the proper precautions. DMSO 99.9% Liquid is sold as a solvent and is an amazing substance that has many uses throughout the world. Dimethyl Sulfoxide is a clear liquid known for its ability to permeate living tissue. It is believed to help stimulate cellular processes.


Daylily people have used it with rust chemicals, BAP, conversion chemicals, etc. to help the chemicals penetrate quicker/easier into the plant. The BIG PRECAUTION IS that anything that is mixed in with the DMSO can and will penetrate into your skin/body if you get it on you so you have to be very careful with it.


[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 26, 2013 6:39 PM CST
This looks like the one Bill uses, but you can get any brand that is 99% pure or 99.9% pure

https://www.google.com/shopping/product/3945682133584693333?...


Thumb of 2013-11-27/tink3472/1b9010

[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
Image
JWWC
Nov 26, 2013 6:43 PM CST
For what it's worth as well, when using DMSO it is not the DMSO per se that is dangerous, it is the possibility that what is in solution with the DMSO can readily gain access as a result.

Also, not all gloves are created equally. It is best to check the chemical rating of the particular gloves prior to use. For example most nitrile and latex gloves are suitable for short or small exposures to DMSO but the solvent will eat through those gloves in a matter of minutes.
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 26, 2013 6:46 PM CST
Bill has in the past also added red food coloring to the Colchicine I would assume so he can see where it is actually at and if it is all absorbed. I don't know if he still uses it or not
Thumb of 2013-11-27/tink3472/2fde7d

[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: Fred Manning
Lillian Alabama

Charter ATP Member Region: Gulf Coast I was one of the first 300 contributors to the plant database! Seller of Garden Stuff Dog Lover Region: United States of America
Ponds Hummingbirder Daylilies Container Gardener Butterflies Birds
Image
spunky1
Nov 29, 2013 8:28 AM CST
Michele, are you ready to start converting, (daylilies that is) I have lots of dip seedlings you could practice on.
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 29, 2013 9:51 AM CST
Sure Fred, I'd LOVE to kill your daylilies Rolling on the floor laughing But seriously, if you want me to try I would love to as there will be some of Kim's I will probably want to convert. If you can find out from Larry where he gets the Colchicine and what type of microscope to use and we shall see what we can do
[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: Betty
Bakersfield, CA
Charter ATP Member I was one of the first 300 contributors to the plant database! Birds The WITWIT Badge Region: United States of America Roses
Irises Cat Lover Daylilies Region: California Garden Ideas: Level 1
Image
Betja
Nov 29, 2013 10:40 AM CST
FANTASTIC!!!

Betty
Name: Becky
Sebastian, Florida (Zone 10a)
Celebrating Gardening: 2015 Daylilies Hummingbirder Butterflies Seed Starter Container Gardener
Charter ATP Member I was one of the first 300 contributors to the plant database! Garden Ideas: Master Level Lover of wildlife (Black bear badge) Birds Ponds
Image
beckygardener
Nov 29, 2013 11:38 AM CST
Michele - It would be very cool to have you document on a new thread on this forum the process!

I, for one, would really like to know what you do and the results!
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
Garden Rooms and Becky's Budget Garden
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
Image
JWWC
Nov 29, 2013 1:57 PM CST
Michele I know Bill Waldrop gets it from Sigma Aldrich. I'd be interested to know what hoops you have to jump through (if any) to get it.
Name: Fred Manning
Lillian Alabama

Charter ATP Member Region: Gulf Coast I was one of the first 300 contributors to the plant database! Seller of Garden Stuff Dog Lover Region: United States of America
Ponds Hummingbirder Daylilies Container Gardener Butterflies Birds
Image
spunky1
Nov 29, 2013 3:13 PM CST
I will talk to Larry when he gets out of the peanut patch in a few weeks. Talked to Cindy a few days ago about there intros and he is busy, busy, busy.
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 29, 2013 3:48 PM CST
JWWC said:Michele I know Bill Waldrop gets it from Sigma Aldrich. I'd be interested to know what hoops you have to jump through (if any) to get it.


I just went to the Sigma Aldrich website and there really isn't any hoops. When you order online (I would imagine the same goes for calling them to order) for the first time they ask about your organization (type), what your job is there, primary business activities, how you will use their products, and of course your name, address, etc. Then they review the info and if you don't pass the review they may call and ask you more question. It's a lot less than I thought they would ask Shrug!

[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: Michele
Cantonment, FL zone 8b
Seller of Garden Stuff Region: United States of America Pollen collector Dragonflies I was one of the first 300 contributors to the plant database! Region: Florida
Birds Butterflies Container Gardener Hummingbirder Garden Ideas: Level 2 Hosted a Not-A-Raffle-Raffle
Image
tink3472
Nov 29, 2013 3:49 PM CST
spunky1 said:I will talk to Larry when he gets out of the peanut patch in a few weeks. Talked to Cindy a few days ago about there intros and he is busy, busy, busy.


Yea I figured he's been busy with the peanuts since he hasn't gotten the website updated yet
[url=www.pensacoladaylilyclub.com]www.pensacoladaylilyclub.com[/url]
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
Image
JWWC
Nov 29, 2013 5:36 PM CST
tink3472 said:

I just went to the Sigma Aldrich website and there really isn't any hoops. When you order online (I would imagine the same goes for calling them to order) for the first time they ask about your organization (type), what your job is there, primary business activities, how you will use their products, and of course your name, address, etc. Then they review the info and if you don't pass the review they may call and ask you more question. It's a lot less than I thought they would ask Shrug!



That's interesting. I stopped when one place had a line about shipping to a residential address. Maybe I will have to pursue it further.

Page 1 of 3 • 1 2 3

« Garden.org Homepage
« Back to the top
« Forums List
« Daylilies forum
You must first create a username and login before you can reply to this thread.

Today's site banner is by ge1836 and is called "Pacific Blue Ice"