Daylily Hybridizing Tips

By Lalambchop1
July 29, 2015

I've been hybridizing for a few years and have been helped along by some of the giants in the industry. Here are some things I've learned...

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Aug 13, 2015 6:23 AM CST
Name: Jessie Worsham
Stockbridge, GA (Zone 8a)
Northwest Georgia Daylily Society
Cat Lover Daylilies Echinacea Region: Georgia Heucheras Hostas
Hybridizer Irises
Thanks Sue for the tip about sub-stage lighting. Being able to take photos is not a priority. I'm thinking about something basic, but with enough magnification to see individual pollen grains. Thanks Maurice for the size info on the pollen. Ebay would probably be a good place to start. I don't want to spend a fortune. I'm hoping to find something under $100.
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Aug 13, 2015 8:35 AM CST
Name: Leslie
Chapin, SC (Zone 8a)
Keeps Sheep Daylilies Hybridizer Garden Photography Cat Lover Hummingbirder
Birds Region: South Carolina Plant and/or Seed Trader Celebrating Gardening: 2015 Garden Ideas: Level 2 Avid Green Pages Reviewer
Jessie,
If you don't want to purchase a microscope you can probaby use the ones at your ag agents office. The Master Gardener volunteers there could help you. Our office has 2-3 very fine microscopes that show more than any the usual gardener could afford.
Leslie

As for me and my house, we will serve the Lord. Joshua 24:15
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Aug 13, 2015 1:53 PM CST
Name: Sue
Ontario, Canada (Zone 4b)
Annuals Native Plants and Wildflowers Keeps Horses Dog Lover Daylilies Region: Canadian
Butterflies Birds Enjoys or suffers cold winters Garden Sages Plant Identifier
Neat that you can do that, Leslie! They're surely better than my microscopes which were, I think, in the $200 to $300 range Canadian, bought new, but that was some years ago. Just out of curiosity, and to illustrate the differences in magnification, I just took some pictures of tet, dip and triploid pollen from the garden, mid-afternoon, at 40X, 100X and 400X with my monocular microscope. I thought I'd got the camera zoom the same for each but when I look at the pics it doesn't look like I did. Still, it should give Jessie an idea of what she needs. I do agree with Maurice that it would be useful to have a simplified way of taking pictures, you never know when you're going to want to show something you've seen to someone else or keep a record. I have to kind of jiggle a digital camera over the eyepiece until I can get things in reasonable focus, and it's not always easy. Anyway, here goes - you can see some of the pollen has burst, not sure if that was the time of day or because I thumped the anthers on the slides to knock the pollen off!:

The dip is 'So Lovely', at 40X, 100X and then 400X
Thumb of 2015-08-13/sooby/7d3dce
Thumb of 2015-08-13/sooby/14700e
Thumb of 2015-08-13/sooby/d99cb5

The tet is 'Sandra Elizabeth, same sizes
Thumb of 2015-08-13/sooby/b5fa13
Thumb of 2015-08-13/sooby/54bc12
Thumb of 2015-08-13/sooby/cce200

The triploid, same sizes, is 'Kwanso' - less consistent pollen sizes it seems to me
Thumb of 2015-08-13/sooby/78ed43
Thumb of 2015-08-13/sooby/54dd62
Thumb of 2015-08-13/sooby/ac067d

Hope this is useful. I had fun, anyway Hilarious!
Edited to add, I should note that I had the sub-stage lighting turned to dim so as not to shine back too much at the camera, which is why some of the pics are a little dark.
Last edited by sooby Aug 13, 2015 1:57 PM Icon for preview
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Aug 14, 2015 5:36 AM CST
Name: Maurice
Grey Highlands, Ontario (Zone 5a)
you can see some of the pollen has burst, not sure if that was the time of day or because I thumped the anthers on the slides to knock the pollen off!:

If the burst pollen was tetraploid and triploid rather than diploid then that is probably misformed/dead/inviable pollen due to problems during meiosis in both those ploidies.
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Aug 14, 2015 1:00 PM CST
Name: Sue
Ontario, Canada (Zone 4b)
Annuals Native Plants and Wildflowers Keeps Horses Dog Lover Daylilies Region: Canadian
Butterflies Birds Enjoys or suffers cold winters Garden Sages Plant Identifier
Based on these pictures it was the tet and triploid, now that you mention it. Thanks, Maurice. So what exactly happens to them, are they just empty skins?
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Aug 14, 2015 4:40 PM CST
Name: Maurice
Grey Highlands, Ontario (Zone 5a)
sooby said:Based on these pictures it was the tet and triploid, now that you mention it. Thanks, Maurice. So what exactly happens to them, are they just empty skins?

You are very welcome. I have not checked the literature but I assume that depending on the chromosome numbers and configurations many simply die before maturing so they could shrink or break open, etc.

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