Micropropagation - Knowledgebase Question

Canberra, In
Question by schmuck_a
August 16, 1999
I would like to know the agar media used to micropropagate kangaroo paws.


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Answer from NGA
August 16, 1999

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Here's a recipe for tissue culture. All the ingredients indicated below can be purchased at the supermarket, pharmacy and/or a health food shop.
1- Two cups of rain water
2- A quarter cup of sugar
3- Fertilizer stock: 1/2 tablespoon all purpose 10:10:10 (N.P.K.) water soluble fertilizer in 1L of water: use one cup of stock for this recipe.
4- Inositol tablets (500 mg): 1/2 tablet
5- Vitamin tablet with thiamine: 1/2 tablet- Any multivitamin tablet may be used.
6- Agar flakes: 4 tablespoons

This is the basic media. Ensure that the pH of medium is always between 5 and 6 using narrow range pH indicator tape. Adjust pH if necessary, with acid e.g. Citric acid or base e.g bicarb soda.

Mix the ingredients in a saucepan and gently boil until the agar has dissolved, stirring continuously to stop the agar sticking and subsequently burning at the bottom of the pan. Dispense into empty glass jars, using a ladle, so that the medium is about 2 cm deep. Cover and process in a pressure cooker. Cook for 15 minutes after the pressure is reached (this will be achieved when the pressure valve starts letting steam out).

Hope this answers your question and gets you on the road to cloning your plants!

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