oneeyeluke said:The good thing about putting in RO water first, is you can see the roots, where in soil you can't. I always put them in water first for roots then transplant in soil.
One of the problems with rooting in water and transferring to soil is the roots grown in water are very brittle and break easily. If the plant is one with tender roots, the transition will not be an easy one. Some cuttings literally grow new roots once they get to soil because the roots grown in water were irreparably damaged in the planting. Roots grown in soil are tough and there isn't a need for transition .
You can tell when a cutting is rooted because it will grow new leaves. If you're impatient, give the cutting a gentle tug. If you are practiced, you can tell the difference between a rooted cutting and a non-rooted cutting by barely touching it. If you can't tell the difference, wait for new growth.