Daylilies forum: Pollen - Freezing and Shipping ideas

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Name: pam
gainesville fl (Zone 8b)
Bee Lover Birds The WITWIT Badge Butterflies Daylilies Enjoys or suffers hot summers
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gardenglory
Aug 29, 2016 6:16 PM CST
So far, I have had no problem with the matchboxes, but I do like those flat tubes. For some reason, I just like the fact of it having a secure lid, as long as its good and dry before putting it in. I have had no luck with pollen mailing. The ice bags are going to be just what I needed to know.
Name: pam
gainesville fl (Zone 8b)
Bee Lover Birds The WITWIT Badge Butterflies Daylilies Enjoys or suffers hot summers
Native Plants and Wildflowers Dragonflies Hummingbirder
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gardenglory
Aug 29, 2016 6:17 PM CST
No, its my fault. I asked her to split it off, because people ask about this alot, and I thought more would see it.
Name: Daniel Erdy
Catawba SC (Zone 7b)
Fruit Growers Permaculture Hybridizer Plant and/or Seed Trader Organic Gardener Daylilies
Region: South Carolina Garden Ideas: Level 2 Garden Photography Herbs Region: United States of America Seller of Garden Stuff
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ediblelandscapingsc
Aug 29, 2016 6:22 PM CST
No worries
­čî┐A weed is a plant whose virtues have not yet been discovered­čî┐
Name: Ed Burton
East Central Wisconsin (Zone 5a)
Hybridizing, Lily Auction seed sell
I was one of the first 300 contributors to the plant database! Tomato Heads Garden Photography Seed Starter Pollen collector Peonies
Hybridizer Hummingbirder Hostas Butterflies Birds Region: Wisconsin
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EdBurton
Aug 30, 2016 6:53 AM CST
I have been using s system for several years also using 1.5 mil centrifuge tubes.
I stuff a wad of cotton in the tube to about a quarter inch from the top, tamped down, I use the blunt end of a Sharpie.
I wrap the tube with a piece of masking tape so I can write down name of plant with pollen inside tube.
I will take the tubes out to the garden and pull off stamens near the base and brush off the pollen to the cotton surface, there is always some pollen left in the groves of the anther if you still want to use it fresh.
It takes on average all six anthers to completely cover the surface of the cotton in the tube.
Tubes are stored in the freezer for use, and can be re froze several times, I found that the fluid on the stigma eventually will make the surface hard, and that tube is essentially used up.
I have set pods in my yard with my pollen that was 5 years old. so it appears pollen stored in a freezer will last a very long time.

Last year I experimented with growers in Georgia and California shipping pollen.
The Georgia grower we shipped early in a doubled walled thermos, and later in the middle of summer through regular mail in bubble wrap envelopes.
What we found is that we set pods both ways, we were worried that the heat would kill the pollen shipped south and west, but even though the temps in both places were in the 90's it didn't seem to matter.
I'm no expert but I believe the tubes with only cotton and pollen stayed dry through the whole process, no stems or anthers to spoil or contaminate pollen with excess moisture in the shipping.
I expanded shipping partners this last season to about 10 people ranging from Canada to Louisiana, that's about the max for my ability to collect and share, with the newest plants even 10 partners is to many.
Northern growers ship to southern growers in the winter, and southern growers ship north in June right before the northern season start.
We have had success setting pods with everyone willing to try using frozen pollen.
In my opinion fresh is best, but with overlapping bloom seasons and not being able to grow every daylily, sharing pollen does make it possible to try new things before buying or owning plants.
I should add that if you can't set a pod on a plant using fresh pollen, frozen pollen will not set one either.
Success rates have vary, but I have pods from stuff I could only dream of before, my partners are enthusiastic seeing the results.

These are the pictures I shared with my partners for collecting and using pollen.
Thumb of 2016-08-30/EdBurton/a6441d
Thumb of 2016-08-30/EdBurton/8aaacd
Thumb of 2016-08-30/EdBurton/5041df
Thumb of 2016-08-30/EdBurton/251408
Thumb of 2016-08-30/EdBurton/cb4b10
Thumb of 2016-08-30/EdBurton/0fbd30
Thumb of 2016-08-30/EdBurton/24cf7b

Ed Burton

seed seller "gramps"
Name: Valerie
Ontario, Canada (Zone 4a)
Daylilies Irises Roses Peonies Butterflies Birds
Bee Lover Region: Canadian Ponds Garden Art Dog Lover Enjoys or suffers cold winters
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touchofsky
Aug 30, 2016 7:01 AM CST
Thank you for that, Ed. Your description and pictures makes the process very clear. I am going to try this next year.
Name: Ed Burton
East Central Wisconsin (Zone 5a)
Hybridizing, Lily Auction seed sell
I was one of the first 300 contributors to the plant database! Tomato Heads Garden Photography Seed Starter Pollen collector Peonies
Hybridizer Hummingbirder Hostas Butterflies Birds Region: Wisconsin
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EdBurton
Aug 30, 2016 7:05 AM CST
Thanks Valerie, let me know how it works for you
Ed Burton

seed seller "gramps"
Name: Christie
Central Ohio 43016 (Zone 6a)
Plays on the water.
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cwhitt
Aug 30, 2016 7:13 AM CST
@JWWC and @EdBurton -- where do you get the tubes from? I was thinking of shipping Amaryllis pollen to someone.
Plant Dreams. Pull Weeds. Grow A Happy Life.
Name: Ed Burton
East Central Wisconsin (Zone 5a)
Hybridizing, Lily Auction seed sell
I was one of the first 300 contributors to the plant database! Tomato Heads Garden Photography Seed Starter Pollen collector Peonies
Hybridizer Hummingbirder Hostas Butterflies Birds Region: Wisconsin
Image
EdBurton
Aug 30, 2016 7:17 AM CST
cwhitt
I buy mine on Ebay, the cheaper the better, look for free shipping for best deals
Ed Burton

seed seller "gramps"
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
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JWWC
Aug 30, 2016 7:35 AM CST
Lol. I'm a cell biologist by trade so I snagged a bag when it showed up on the give away shelf in the department.

They're pretty readily available though from sources through Amazon or companies like USA Scientific.
Name: Steve Todd
Illinois (Zone 5b)
Region: Illinois Plant and/or Seed Trader Daylilies Enjoys or suffers cold winters
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Ahead
Aug 30, 2016 7:57 AM CST
I get mine from James....lol.
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
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JWWC
Aug 30, 2016 9:50 AM CST
This is my go to method for storing pollen.

1. Harvest the pollen. I usually just pull the entire stamen and anther off of the bloom. If it is something I really want and I know, for example, it is going to rain, then I will take them even when the anthers are closed. I bring them inside and remove the anthers from the stamen and leave the pollen sacs to open and dry on a piece of printer paper with the cultivar name written on it. I leave them on the stove top because it is flat and I can turn on the small light on the fan. Warm temps and some light and they usually open right up.

2. Save the pollen. Between 8 and 24 (but when busy I've left them out to 72) hours I will place all the anthers for a given cultivar into a 2 ml centrifuge tube and then flick the side of the tube several times to release the pollen.

3. Remove the spent anthers. I don't save the anthers. I know some do but for me it was a recipe for failure. I typically just gently tap the tube over a waste container and they come out pretty readily. You could use forceps but I was weary about cross contaminating my pollen. Yes, the retentive scientist got the better of me there!

4. For the love of all things good in the world label your tubes.

5. Freeze. I put them into boxes and stick them in the freezer.

A couple notes at this point: I try to have multiple tubes of each pollen because I'm paranoid and I have been known to stick a tube in my pocket and forget about it. I also will only put 1-2 blooms worth in a tube, generally if I am putting 2, I just repeat the process rather than try and have 12 anthers in at once.

To use the pollen:

1. I make a list of everything I want to use that day, and then pull them out of the freezer and put them in a tube rack that I keep at room temp to avoid excess condensation.

2. Normally by the time I am ready to go, the pollen tubes are warm enough to use. The enemy is condensation on the inside of the tube. You don't want to take a freezing cold tube out into super humid air and open it. If that's a concern I would let it come to temp for about 5 minutes.

3. I use toothpicks to dab the pollen. I buy the ones that are flattened. One end is rounded and the other pointed usually. Then I just gather a small amount on the tip of the toothpick and place it on the bloom. Usually I will keep using the same toothpick for the same pollen unless I notice that I've gotten it wet with dew or whatever else is floating around the garden that day.

4. Back in the freezer. I just put the tubes back in the box and go on with my day.

Brief notes - I try to keep my tubes in the shade while I am outside just so they don't get extremely hot. This is mostly a concern when it's already rather warm in the morning. Also, if it is really humid, make sure the tubes warm before opening. Condensation forming on the inside of the tube will quickly ruin your pollen. Or if you are just unlucky like me and a drip of sweat falls straight into the tube... D'Oh!
Name: Betty
Bakersfield, CA
Charter ATP Member I was one of the first 300 contributors to the plant database! Birds The WITWIT Badge Region: United States of America Roses
Irises Cat Lover Daylilies Region: California Garden Ideas: Level 1
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Betja
Aug 30, 2016 9:54 AM CST
I found a great deal from USA Scientific and I now get my tubes from them -- $13.95 for 500!

http://www.usascientific.com/s...

But you have to order that particular item # because the others are more expensive and this item # doesn't always seem to appear when searching for tubes.

Betty
Name: Steve Todd
Illinois (Zone 5b)
Region: Illinois Plant and/or Seed Trader Daylilies Enjoys or suffers cold winters
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Ahead
Aug 30, 2016 10:31 AM CST
Betty,

That's a great price! But mine are still free....lol.

Name: Betty
Bakersfield, CA
Charter ATP Member I was one of the first 300 contributors to the plant database! Birds The WITWIT Badge Region: United States of America Roses
Irises Cat Lover Daylilies Region: California Garden Ideas: Level 1
Image
Betja
Aug 30, 2016 10:52 AM CST
Hilarious!
Betty
Name: Daniel Erdy
Catawba SC (Zone 7b)
Fruit Growers Permaculture Hybridizer Plant and/or Seed Trader Organic Gardener Daylilies
Region: South Carolina Garden Ideas: Level 2 Garden Photography Herbs Region: United States of America Seller of Garden Stuff
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ediblelandscapingsc
Aug 30, 2016 1:34 PM CST
Great info everyone, I look forward to freezing some pollen this coming season Thumbs up
­čî┐A weed is a plant whose virtues have not yet been discovered­čî┐
Name: Stan
Florida Panhandle (Defuniak Sp (Zone 8b)
Region: Florida Region: Gulf Coast Enjoys or suffers hot summers Daylilies Lilies Keeps Horses
Dog Lover Garden Photography Butterflies Hummingbirder Birds Bee Lover
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GaNinFl
Sep 1, 2016 6:25 AM CST
I agree Thanks for the tips. Anxious to get started next year...
Stan
(Georgia Native in Florida)
http://garden.org/blogs/view/G...
Name: pam
gainesville fl (Zone 8b)
Bee Lover Birds The WITWIT Badge Butterflies Daylilies Enjoys or suffers hot summers
Native Plants and Wildflowers Dragonflies Hummingbirder
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gardenglory
Sep 1, 2016 7:35 AM CST
Im going to try the cotton. That is new to me. Im feeling a bit like an old dog, so why not try a new trick.

Ive yellow stared all of this to save. I dont know how to find my stars yet, but at least I know they are there.
[Last edited by gardenglory - Sep 1, 2016 7:38 AM (+)]
Give a thumbs up | Quote | Post #1259425 (17)
Name: Stan
Florida Panhandle (Defuniak Sp (Zone 8b)
Region: Florida Region: Gulf Coast Enjoys or suffers hot summers Daylilies Lilies Keeps Horses
Dog Lover Garden Photography Butterflies Hummingbirder Birds Bee Lover
Image
GaNinFl
Sep 1, 2016 9:19 AM CST
@gardenglory

Pam,

If you select your profile and scroll down to the "some info about" area you will see starred pages...

Stan
(Georgia Native in Florida)
http://garden.org/blogs/view/G...
[Last edited by GaNinFl - Sep 1, 2016 9:22 AM (+)]
Give a thumbs up | Quote | Post #1259504 (18)
Name: pam
gainesville fl (Zone 8b)
Bee Lover Birds The WITWIT Badge Butterflies Daylilies Enjoys or suffers hot summers
Native Plants and Wildflowers Dragonflies Hummingbirder
Image
gardenglory
Sep 1, 2016 9:37 AM CST
Thank you, I finally found it. Not in a hundred years would I have found that without instruction Confused
Name: Daniel Erdy
Catawba SC (Zone 7b)
Fruit Growers Permaculture Hybridizer Plant and/or Seed Trader Organic Gardener Daylilies
Region: South Carolina Garden Ideas: Level 2 Garden Photography Herbs Region: United States of America Seller of Garden Stuff
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ediblelandscapingsc
Sep 1, 2016 11:22 AM CST
I too am going with the cotton idea. Thumbs up
­čî┐A weed is a plant whose virtues have not yet been discovered­čî┐

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