Gardening Ideas forum: Auxin injections? A truly hypothetical idea.

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Name: Ian
Brooklyn, NY, 11231 (Zone 6b)
Araceae über alles!
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Hagbard
Sep 22, 2017 10:52 AM CST
So this is an *IDEA* that I've been toying with for some time, and will be attempting to implement over the weekend. I'll keep you all posted on the success or failure of the experiment; I have no idea what will come of it but what the hell, worth a try, yes?

So as you might know if you're as much a dork as I, auxins are the hormones within a plant that are released in various quantities in various areas of the plant. They control everything from phototropism, geotropism, and hydrotropism to the allocation of growth resources (whether the plant puts its "effort" into new growth, larger leaves, longer stems, more roots, etc) to cellular change on the molecular level - including the weakening of cellulose which can accelerate the lengthening of cells, as well as their relative flexibility.

Rooting hormone contains two of the principle auxins that control, among other things, growth & the weakening of the cellulose in cell walls - NAA and IBA. Most other auxins are not, as such, readily available, but that's OK. Now we're about to get into the theoretical stuff.

Q: Ian, what would happen if you made three different strengths of auxin solution, and used a hypodermic needle to inject them into different areas of test plants (to start with, Golden Pothos, since mine are so large and I can't *GIVE* cuttings away anymore)?

A: I have no freaking clue, but it will be interesting to find out! That's how experimentation works, yes?

So since auxins are not water soluble, I'm going to buy three little airplane bottles of the purest spirits that I can - auxins dissolve just fine in ethanol. I'm thinking that vodka is the way to go, since I don't have access to proper, lab-grade ethanol. I'll heat it until it's a little warm to maximize absorption, and then mix in rooting hormone until I reach saturation, closing the bottle and letting any undissolved hormone sink to the bottom.

Then I'll make three dilutions of the stuff with water, since a little bit of this hormone is incredibly powerful: 1:10, 1:30, and 1:100. I'll use the tiny hypodermic needle and glass syringe that I found buried in a pile of debris in a tuberculosis sanatorium that had been derelict for 50+ years, which I will first boil to sterilize, and then I'll have a go at injecting various parts of various pothos (I have 9 rooted at this moment), noting my progress as I go. And I will, of course, keep you all apprised of any results, assuming I achieve any!

Well, it is the ideas forum, and this is an idea. Just don't know if it's a good one - but we'll see!
Name: josephine
Arlington, Texas (Zone 8a)
Hi Everybody!! Let us talk native.
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frostweed
Sep 22, 2017 11:19 AM CST
Ian, you are amazingly full of original ideas, good luck to you with your experiments. Smiling Hurray!
Wildflowers are the Smiles of Nature.
Gardening with Texas Native Plants and Wildflowers.
Name: Karen
NM (Zone 7b)
Region: New Mexico Critters Allowed Region: Arizona Xeriscape Greenhouse Annuals
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plantmanager
Sep 22, 2017 11:51 AM CST
I don't know either, but it's a very interesting experiment, and I'll look forward to your findings.
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Name: Frenchy
Falls Church, VA (Zone 7b)
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Frenchy21
Sep 22, 2017 12:10 PM CST
Ian it sounds fascinating! Can't wait to see what happens.Keep your ideas and experiments coming Thumbs up Thumbs up
Name: Ian
Brooklyn, NY, 11231 (Zone 6b)
Araceae über alles!
Houseplants Container Gardener Foliage Fan Aroids Region: New York
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Hagbard
Sep 22, 2017 6:48 PM CST
Ready for the weekend!

Thumb of 2017-09-23/Hagbard/4a8b7b

Name: Joanna
North Central Massachusetts (N (Zone 5b)
Life & gardens: make them beautiful
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joannakat
Sep 22, 2017 10:08 PM CST
Hagbard said:Ready for the weekend!

Thumb of 2017-09-23/Hagbard/4a8b7b



If this weren't a gardening forum, that would look very, very strange! Blinking
AKA Joey.
Name: Frenchy
Falls Church, VA (Zone 7b)
Container Gardener Dog Lover Houseplants Plant Lover: Loves 'em all! Tomato Heads Hostas
Tropicals Annuals Foliage Fan Aroids Cactus and Succulents Sempervivums
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Frenchy21
Sep 22, 2017 11:46 PM CST
Ian take care not to stick yourself with that needle - looks wicked Sticking tongue out
Name: Ian
Brooklyn, NY, 11231 (Zone 6b)
Araceae über alles!
Houseplants Container Gardener Foliage Fan Aroids Region: New York
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Hagbard
Sep 23, 2017 1:30 AM CST
joannakat said:

If this weren't a gardening forum, that would look very, very strange! Blinking


Well it's not like anybody's going to drink Georgi vodka! But it is useful for dissolving ethanol-soluble matter that cannot be dissolved in water :P Hypos and needles are pretty commonplace for me, since I photograph abandoned insane asylums, sanatoriums, and so on for a living. And the rooting hormone... well that could just be where I hide my drugs!

Frenchy21 said:Ian take care not to stick yourself with that needle - looks wicked Sticking tongue out


I'd be more worried about damaging it. The glass syringe is over 60 years old - back from when they made things to be reused, and not tossed off in the rubbish when used once.
Name: Joanna
North Central Massachusetts (N (Zone 5b)
Life & gardens: make them beautiful
Million Pollinator Garden Challenge Vermiculture
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joannakat
Sep 23, 2017 10:23 AM CST
Hagbard said:

I'd be more worried about damaging it. The glass syringe is over 60 years old - back from when they made things to be reused, and not tossed off in the rubbish when used once.


That's amazing. Should be in a glass case by itself.

Just in case anyone is interested, you can get syringes in any pharmacy these days. I'm not sure about the needles, but they are (at least in MA), required by law to give them away without charge. They are the disposable type, but if you're using them for gardening purposes, you can reuse them.
AKA Joey.
Name: josephine
Arlington, Texas (Zone 8a)
Hi Everybody!! Let us talk native.
Native Plants and Wildflowers Organic Gardener Million Pollinator Garden Challenge Butterflies Garden Ideas: Master Level Forum moderator
I was one of the first 300 contributors to the plant database! I helped plan and beta test the plant database. Charter ATP Member Plant Identifier Birds Cat Lover
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frostweed
Sep 23, 2017 11:49 AM CST
Good info Joanna!! Smiling Thank you.
Wildflowers are the Smiles of Nature.
Gardening with Texas Native Plants and Wildflowers.

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