I'd say they were partially calloused as they had been cut within 3-7 days of being put into the water (some of the trees they came from were not in perfect shape to begin with and were partially dehydrated when cut - beggars can't be choosers). I did question whether putting them in water at this point was the right decision, so I didn't put them all in the water - I didn't want to ruin all my cuttings if my thought process was off!
Using the same thought process, I pulled a few out of the water last night and dipped them into rooting hormone and will wait for them to callous. A couple that felt like they could use another day or two, I left in.
I will say that the centers of the cuttings left in the water have softened some, while the edges have stayed relatively firm. The overall cuttings have firmed even though some of the wrinkles remain.