Lilies forum: Tetraploid OT, LO, LA Seed Germination?

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Ontario, Canada (Zone 6a)
Phenolic
Jul 13, 2015 12:08 AM CST
Since lilies from distant crosses have a high tendency to produce 2n gametes, have any experienced hybridizers produced viable tetraploid or triploid seeds from any crosses using diploid or tetraploid OT/LO/LA/etc. parents? It is possible to germinate the resulting seeds naturally and without any laboratory techniques like embryo rescue/culture?
Name: Øystein Hermansen
Østfold,Norway (Zone 5b)
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grapus
Jul 13, 2015 9:15 AM CST
I have best germination sowing 4n seeds having them in a cup of water for 2-3 days changing water 2 times a day. It's possible to get seeds when crossing 4n OT x 4n trumpets, 2n OT x 2n trumpets, LO x 4n OT, LO x 4n orientals, LA x (4n?) asiatics. The old OTs have poor fertility but newer hybrids can produce many seeds in every pods. My best advice is to use mixed pollen from 10 different lilies.
Ille bra,se.
Ontario, Canada (Zone 6a)
Phenolic
Jul 14, 2015 2:20 PM CST
Thanks for the reply, Øystein! Crossing lilies with a goal in mind is rather different from the pollen dabbing I've been doing, I have found.

I've done some literature reading for lily cytogenetics, and most of the authors cultured their embryos. So, I've been confused about distant crosses like OT's, LA's, and LO's.
Name: Joe
Long Island, NY (Zone 7a)
Lilies Region: New York Plant and/or Seed Trader Seed Starter Garden Ideas: Level 1
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Joebass
Jul 14, 2015 2:58 PM CST
From what I've read, it's best to put 4N pollen on triploids and it may be possible to get seeds. Sometimes I try to think with goals like for instance I have a beautiful LO called Pink Heaven which is supposedly 4N. So I figured that if I put some of that pollen on Easter Morn (trumpet x Longiflorum) I may get something due to a common grandparent. We'll see what happens in the fall.
Name: Tracey
Wisconsin (Zone 5a)
Forum moderator Hybridizer Tomato Heads Pollen collector Hosted a Not-A-Raffle-Raffle Cat Lover
I was one of the first 300 contributors to the plant database! Plant Database Moderator Charter ATP Member Garden Photography Seed Starter Region: Wisconsin
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magnolialover
Jul 14, 2015 5:06 PM CST

Moderator

Lilium genetics are funny like that. But never say never, you never know when you'll get seed. But a good goal to me, would not only come up with something nice to look at but also strong genetically, long lasting and virus resistant and or tolerant. So putting those traits in the so called "mix" is a good thing.
Tracey
Ontario, Canada (Zone 6a)
Phenolic
Jul 14, 2015 10:36 PM CST
Joe, is that what "bridging" is?

Tracey, lily beetle and virus tolerance are on the top of my list of traits to breed for! I'm building up my pool of knowledge and collecting lilies for genetic diversity in the meanwhile.

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