Biosphere 2 in Arizona started as an experiment to see if life could be sustained in a closed, sealed system--such as would be necessary on Mars. It basically failed at that, twice, and in some interesting ways.
It now belongs to the University of Arizona, and experiments continue in this unique controlled environment. It's no longer sealed.
It has various habitats, including rain forest, savanna, desert and ocean.
Right now I think they have some on-line educational programs. When we can all get out again, I highly recommend a visit and a tour. It's quite interesting. Sure made me appreciate Biosphere 1!
https://biosphere2.org/