I had some leaves (10 total) sent out for FrMV testing and got the results back today (The results are attached to this email.). I also sent in some leaves from a friends plants in Fl. The plants that I thought, would come up positive did; Thai red orange with leaf mottling and "splash" flowers and Marble queen which is a known "splash" flower sold out of Thailand were positive for FrMV. What I found interesting though is that a seedling, which I sent as the control, also came up positive. I have never cut on this plant, but it has been in the vicinity of some virus plants. My thoughts are that it is a false positive and the test center screwed up somehow (going to ask for a re-test) or the virus is somehow vectored by means other than just sap transfer as the Thai Orange red and other suspect virus plants (I say suspect as they have not been tested but do show the symptoms) were growing in the same area as the seedling. I would like your thoughts on this please.
Thanks,

Davie Brooks II
Yuma, AZ

October 22, 2010

Davie Brooks II
6580 E County 3rd Street
Vuma, AZ 85365
(928) 580-4222

Customer Number: TS1023
Job Number: J1111
Samples Received: October 21, 2010

Report of Testing Result for Your Samples

The 10 sample has been tested for the presence of TMV and FrMV. The tests are performed based on the protocol provided by you. The test results are as following:

Virus
Abbreviation
Results
Tobacco Mosaic Virus TMV Negative
Frangipani Mosaic Virus FrMV Sample Plumeria Rubra “Thai Orange Red”-1, Plumeria Rubra “Thai Orange Red”-2,
Plumeria Rubra “Thai Orange Red”-3, Plumeria Rubra “Marble Queen” – 1
Plumeria Rubra “Marble Queen” – 2,
Plumeria Rubra “Marble Queen” – 3,
Plumeria Rubra “Lava Flow” , and
Plumeria Rubra “Seedling” are Positive
Sample Plumeria Rubra “King Kyle” – 1 and Plumeria Rubra “King Kyle” – 2 are Negative

Sample Plumeria Rubra “Thai Orange Red”-1, Plumeria Rubra “Thai Orange Red”-2,
Plumeria Rubra “Thai Orange Red”-3, Plumeria Rubra “Marble Queen” – 1
Plumeria Rubra “Marble Queen” – 2, Plumeria Rubra “Marble Queen” – 3,
Plumeria Rubra “Lava Flow” , and Plumeria Rubra “Seedling” are Positive for FrMV.
Sample Plumeria Rubra “King Kyle” – 1 and Plumeria Rubra “King Kyle” – 2 are Negative for FrMV. All the samples are negative for TMV. For the detailed information, please refer to the following page for the testing data on the samples submitted.

Thank you for using ACD Testing Services. Please do not hesitate to contact us if you have any questions.


Sincerely yours,



John Q. Xia, Ph.D.
Testing Services, AC Diagnostics, Inc.





Testing Results of the Samples Submitted*

Job Number: J1111, 10/22/10

Samples
Controls

Tests Plumeria Rubra “King Kyle”–1 Plumeria Rubra “King Kyle”-2 Plumeria Rubra “Thai Orange Red”-1 Plumeria Rubra “Thai Orange Red”-2 Plumeria Rubra “Thai Orange Red”-3
Neg.
Posi.
FrMV 0.139 0.113 0.446 0.372 0.887 0.107 3.433
TMV 0.086 0.095 0.120 0.127 0.094 0.103 3.697

Tests Plumeria Rubra “Marble Queen” – 1 Plumeria Rubra “Marble Queen” – 2 Plumeria Rubra “Marble Queen” – 3 Plumeria Rubra “Lava Flow” Plumeria Rubra “Seedling”
FrMV 0.656 0.626 0.496 0.492 0.441
TMV 0.112 0.110 0.133 0.146 0.108

*Any OD numbers of 3 times of the negative are considered positive.

So far all I can say is 'very interesting' - others may have more input. Thank you for posting this, Davie.