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Sep 2, 2016 2:24 PM CST
Name: Maurice
Grey Highlands, Ontario (Zone 5a)
@Deryll,
There are several different ways to clone a plant.

The simplest is to thoroughly clean the crown of a daylily fan. Remove all the roots and all the leaves and then to cut the crown into quarters, eighths, sixteenths, and so on (basically into small pieces that still have the ability to produce axillary buds but no longer have their primary growing point).

Now those pieces need to be placed in a gel (agar or similar plant gels not animal gelatin) with minerals and some plant hormones. The plant hormones are not that much different from the plant hormones used to get roots to form (the plant hormones that are present in rooting gels and powders).

The result is that each piece of the original crown produces a handful of new fans.

The alternate way is to take a small piece of a daylily. Place that in the agar gel with minerals but this time with a different mixture and concentration of plant hormones to get the piece of tissue to lose its identity and then become a growing point to produce a new fan.

The temperature to keep the cultures at would be any reasonable temperature at which daylilies grow. I would think that 25C or 78F would be close to optimum.
Maurice

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