Viewing post #2573714 by admmad

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Aug 12, 2021 2:14 PM CST
Name: Maurice
Grey Highlands, Ontario (Zone 5a)
If you become a member at archive.org you can read many books online by "borrowing" them for one hour or sometimes 14 days.

This book, Plant Cell and Tissue Culture: Principles and Applications. Ed. By W. R. Sharp, P.O. Larsen, E.F. Paddock, and V. Raghaven. Columbus: Ohio State University Press. is available at archive.org for reading (and downloading to read on your home computer with restricted access).

I would direct you to this paper by Krikorian & Kann, but unfortunately only the abstract is available online - provided below,

ANCYMIDOL SUPPRESSES HEIGHT AND ENHANCES MULTIPLICATION IN VITRO OF DAYLILY (HEMEROCALLIS)
Propagation of Ornamental Plants 3(1): 11-19, 2003
Abraham Krikorian* and Robert Kann

Abstract
The anti-gibberellin ancymidol was tested on three daylily hybrid clones to evaluate its ability to foster internode shortening of in vitro cultured shoots. At concentrations as low as 0.04 µM shoot height was reduced to ~ 50% of controls after six weeks exposure. Leaves of 'dwarfed' shoots or plantlets were 2 to 10 times shorter than control leaves and were more typical of leaves produced by mature, rather than juvenile plants. "Ancymidol leaves" showed overlapping bases and the "keel" or central dorsal ridge on the abaxial side of the leaves became more prominent and assumed a fan-like appearance. Because the leaves were more concave on the adaxial surface, they presented a "cupped" or canaliculate, longitudinally grooved morphology. Roots produced by ancymidol-grown plants were thicker and more fibrous than control roots, again a feature of mature phenotype. Shoots grown for protracted periods (ca. 14 to 16 weeks) showed a substantial increase in shoot production. At concentrations below 0.39 µM there was no effect on multiplication but as the ancymidol concentration increased, multiplication increased. There were differences in responsiveness to ancymidol among the clones for bud multiplication. Effects of ancymidol on phenotype and multiplication were reversible.

The reason I would direct you to research on anti-gibberellins is because gibberellic acid affects many perennials in the opposite way you would like (i.e. it delays flowering). This paper (I think since I have not been able to obtain it) appears to provide some evidence that ancymidol may act against gibberellic acid in daylilies to shorten their juvenile period and produce earlier flowering (under appropriate circumstances).
Maurice

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