Well, I wouldn't leave it out very long without at least putting it temporarily back in the fridge. My practice is to avoid exposing my test tubes and pollen to direct sun. As soon as I finish pollenating for the morning, I put it in the refrigerator to chill/normalize and then refreeze that evening. If I plan to use the same pollen again within the next few days, I'll just hold it in the fridge for those few days and then refreeze. I keep my time intervals as short as possible in all instances. But I have held thawed pollen in the refrigerator for 3 or 4 or 5 days and used it with reasonable results (to me, anyway) on a few of my Divison VI crosses. But no one should intrepret that as a broad base statement of standard practice that applies to all Divisions and Cultivars. There are so many variables that enter in at this point and beyond that one would never know if the results are accurate or accidental or whatver.
So, about 5 days refrigerated is as far as I've gone with thawed pollen. I have in a couple instances, however, used pollen again the second season (thawed and then refrozen) with seemingly acceptable results but I could never be absolutely sure my results were what I intended or what caused them to be what they ended up being.. For instance, pod filling, seed generation on some was only about 20 % or less and lots of chaff--certainly not the best but I can't automatically blame pollen virility with so many other variables. So many unknowns to tease us!
