I learned a good tip today on working with frozen pollen from Tom Bruce of Carolina Daylilies. He said he uses frozed pollen extensively. Since you take the risk of introducing moisture into your pollen tube every time you open it, he only puts one or two anthers into each tube. What a great idea.
I also wanted to post a pic of what I call the "Jeffcoat method" of saving pollen. Jim and Peg Jeffcoat showed me this one last year. They don't work with frozen pollen but use an old fashioned ice cube tray to sort their fresh anthers. They keep the tray in the refrig and refresh the stock of each variety as new blooms appear. Couple that with reverse tweezers and you're good to go.
Leslie
As for me and my house, we will serve the Lord. Joshua 24:15