Daylilies forum: Storing and freezing pollen questions ???

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Name: Becky
Sebastian, Florida (Zone 10a)
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beckygardener
Nov 14, 2015 11:51 PM CST
I received some centrifuge tubes with caps and the box to store them in today.

I read this article:
http://garden.org/ideas/view/Cat/1978/Great-Way-To-Save-Poll...

How would I go about saving the pollen? Do I use cotton balls cut up and stuffed down into the tubes or what?

Do I let the pollen dry out first before adding to the tubes?

Then put them in the freezer and forget about them until I want to use them?

Any advice or suggestions would be greatly appreciate! Thank you in advance. Thank You!
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Name: Tim
Omaha, NE (Zone 5b)
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tcmfish
Nov 15, 2015 7:43 AM CST
Also interested!
Name: Fred Manning
Lillian Alabama

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spunky1
Nov 15, 2015 7:53 AM CST
I remove the pollen sacs from the stamens and air dry for about 12 hours on a post-it-note with name of the flower. I then put it in the tube with nothing added, make sure the tube is closed tight, put it in the freezer and forget about it until you want to use it.
Name: Becky
Sebastian, Florida (Zone 10a)
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beckygardener
Nov 15, 2015 7:54 AM CST
Ok, I got desperate because I have 2 blooms on Marilyn Morss Johnson today and want to save that pollen. So I found this article: http://www.hostalibrary.org/misc/pollen/

Does sound like it would work!

Anyone care to comment or explain how you store and freeze pollen?
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
Garden Rooms and Becky's Budget Garden
Name: Becky
Sebastian, Florida (Zone 10a)
Celebrating Gardening: 2015 Daylilies Hummingbirder Butterflies Seed Starter Container Gardener
Charter ATP Member I was one of the first 300 contributors to the plant database! Garden Ideas: Master Level Lover of wildlife (Black bear badge) Birds Ponds
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beckygardener
Nov 15, 2015 7:54 AM CST
Thank you, Fred! Thumbs up
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
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Name: Char
Vermont (Zone 4b)
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Char
Nov 15, 2015 8:58 AM CST

Moderator

You just have to love post-it notes! Big Grin I couldn't live without them. I do the same as Fred, then put one or two anthers in a gel cap. The caps are then placed in a ziploc snack size baggie with the name written on the top edge. An empty baggie box is kept in the freezer for storing and the baggies are placed in the box like file folders with the name at the top making it easy to flip through and find the bag of pollen I want to use.
Name: James
South Bend, IN (Zone 5b)
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JWWC
Nov 15, 2015 12:38 PM CST
I harvest the pollen sacs (usually early in the morning) and if they are not already open, they go under a light in the kitchen as they are allowed to dry. I haven't used post-it notes but I typically have a piece of paper with the name written on it and a small pile of pollen sacs drying. I can fit about 12 different flowers worth onto a single page.

When I come home from work I will write the name on the top of a centrifuge tube, place the sacs into the tube, then flick it a few times to liberate the pollen. I remove the sacs (this is easier than you would think) and place the tube in the freezer. Eventually I will print a batch of labels for the top of the tubes (green for tet and orange for dip) because I have had my fair share of writing disappear on me.

When I go to pollinate I will use a small toothpick with a flat end to collect some pollen and place it on the stigma.
Name: Alex
Warren, VT- Green Mtns. (Zone 4b)
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ARoseblush
Nov 15, 2015 3:00 PM CST
Becky: Hi girl. I use a small centrifuge box filled with centrifuge tubes that has a top affixed to the tube. Basically, I collect the pollen. Place it on a paper plate that I label to let it dry for 7 hrs. I place the pollen in the tube that I stuff with cotton for ease of using the pollen down the road. I then, place the tube back in the pollen box after I label it with a piece of masking tape, and pop the pollen box with the tubes back into the freezer. It's easy, quick, and very efficient.

Th centrifuge boxes can be purchased on the LA. They are very compact and hold approx. 100 tubes.
Name: James
South Bend, IN (Zone 5b)
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JWWC
Nov 15, 2015 4:19 PM CST
I will say as someone who uses microfuge tubes and freezer boxes on a daily basis for work (not just for daylilies) the 9x9 dividers are easier with the 1.5 mL tubes. The others don't leave enough room for the caps and end up being too crowded.

If you're really pressed for space there are smaller 0.5 mL tubes but I'm not sure if there are different dividers that will let you store more in a box.
Name: Mayo
The Netherlands, Europe (Zone 9a)
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Mayo62
Nov 17, 2015 1:01 PM CST
I have never done this, and so haven't been paying enough attention to the info regarding it yet Thumbs down , but I read in another post that there are some important do's and don't when thawing the pollen..

If you do it the wrong way you would destroy the pollen because you would get condensation on the inside of the tube (I think??) Shrug!
I'm not sure whether it was about thawing inside the fridge instead of outside the fridge, or remembering to take the cap of the tube...? Confused



Mayo
a DL flower a day keeps the doctor away
Name: Becky
Sebastian, Florida (Zone 10a)
Celebrating Gardening: 2015 Daylilies Hummingbirder Butterflies Seed Starter Container Gardener
Charter ATP Member I was one of the first 300 contributors to the plant database! Garden Ideas: Master Level Lover of wildlife (Black bear badge) Birds Ponds
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beckygardener
Nov 17, 2015 3:20 PM CST
Mayo - You must have been reading my mind!!! I was just about to post to ask this question.

How do you thaw and dry the pollen once you remove it from the freezer?
What lies behind us and what lies before us are tiny matters, compared to what lies within us.
Garden Rooms and Becky's Budget Garden
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
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JWWC
Nov 17, 2015 3:41 PM CST
I put the tubes containing pollen I will use in a microfuge rack like this:


Thumb of 2015-11-17/JWWC/637e48

Mine is dark green because that is what I found on the 'free' shelf in the department one day.

I take that and my note pad with the crosses I intend to make, out into the yard and go to work. It takes very little time for the pollen to 'thaw' and in reality I don't think it needs to be a certain temp before you use it, but it will need to thaw before it is activated. I've not had any trouble doing it this way. I am careful to keep the rack in the shade.

When I'm done I close the tubes and put them back in the freezer.
Name: James
South Bend, IN (Zone 5b)
Hostas Enjoys or suffers cold winters Birds Seed Starter Annuals Region: Indiana
Region: United States of America Dog Lover Daylilies Container Gardener Plant and/or Seed Trader
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JWWC
Nov 17, 2015 3:43 PM CST
Mayo62 said:I have never done this, and so haven't been paying enough attention to the info regarding it yet Thumbs down , but I read in another post that there are some important do's and don't when thawing the pollen..

If you do it the wrong way you would destroy the pollen because you would get condensation on the inside of the tube (I think??) Shrug!
I'm not sure whether it was about thawing inside the fridge instead of outside the fridge, or remembering to take the cap of the tube...? Confused



Mayo


As long as I dry the pollen before it goes in the tube I have had no trouble with frozen pollen. Granted it is not nearly as humid here. If I remember the post correctly they problem is that if you take a cold tube into the humidity and open it up immediately, condensation can form when the humid air hits the cold inside of the tube. The solution is to let the tube come to temp before opening.

Name: Mayo
The Netherlands, Europe (Zone 9a)
Region: Europe Cat Lover Daylilies Irises Dog Lover Hellebores
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Mayo62
Nov 17, 2015 4:55 PM CST
I agree
Ah, yes that was it Thumbs up
a DL flower a day keeps the doctor away

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