clockworkhawkins said:
I was under the impression that the 2n gamete developed via pollen during the first stages of bud development? Correct me if im wrong but if the pollen develops as normal it produces a haploid. but are you saying you take the haploid pollinate the flower then crank the heat to 110 for the first half hour?
Natural polyploids may be produced by different methods. They may be produced by 2n gametes or by somatic chromosome doubling.
2n gametes may be male or female and may be produced by specific mutations or by environmental effects (temperature shocks). They may be produced at two different stages of meiosis in flower buds.
Somatic chromosome doubling may be produced by environmental effects (temperature shocks) at early stages of shoot apical meristem or axillary meristem development in vegetative buds.
If you pollinate a diploid flower with normal pollen and know the time at which the embryo undergoes its first division and apply a temperature shock then you sometimes may cause somatic chromosome doubling and that seed may develop into a tetraploid. The timing would not be in the first half hour; it is not known for daylilies.